Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE130065

GEO first seen 2019

CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics

Organism
Homo sapiens; Mus musculus
Samples
5
Type
Expression profiling by high...
Submitted
2019-04-19

High-throughput single-cell assays increasingly require special consideration in experimental design, sample multiplexing, batch effect removal, and data interpretation. Here, we describe a lentiviral barcode-based multiplexing approach, CellTag Indexing, which uses predefined genetic barcodes that are also heritable, enabling cell populations to be tagged, pooled, and tracked over time in the same experimental replicate. We demonstrate the utility of CellTag Indexing by sequencing transcriptome...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Samantha A MorrisChuner Guo
Reused by

Deep data QC

metadata only · no data-level QC for this type

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
bulk-RNA-seq
Organism
Mus musculus; Homo sapiens
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
5 / 5 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 137969228422 reported
total reads 1036061167 reported
supplementary file types CSV, MTX, TSV, TXT reported
QC cost 6 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently