Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE131155

GEO first seen 2019

Comprehensive single cell transcriptome lineages of a proto-vertebrate

Organism
Ciona intestinalis
Samples
23
Type
Expression profiling by high...
Submitted
2019-05-13

Studies of ascidian (sea squirt) embryos have highlighted the importance of cell lineages in animal development for over 100 years. As simple proto-vertebrates, they are also used to explore the evolutionary origins of novel cell types, such as cranial placodes and neural crest in vertebrates. To build upon these efforts we have determined comprehensive single cell transcriptomes of Ciona intestinalis throughout embryogenesis. More than 90,000 cells from 10 different developmental stages were...

Provenance — who produced it, who reused it

Linked to 4 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Chen CaoMike S Levine
Reused by

3 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Ciona intestinalis
Metrics (value · how obtained)
n content pct 0.006 measured
pct q20 bases 99.2 measured
pct q30 bases 98.4 measured
gc content pct 50.5 measured
mean read length 26 measured
mean base quality 38.3 measured
adapter content pct 0.42 measured
duplication rate pct 4.13 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 98.4 measured ×1 100%
mean base quality 38.3 measured ×0.6 100%
adapter content pct 0.42 measured ×0.4 100%
duplication rate pct 4.13 measured ×0.4 100%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0