Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE131778

GEO first seen 2021

Single cell analysis of smooth muscle cell phenotypic modulation in vivo during disease in mice and humans [human scRNA-seq]

Organism
Homo sapiens
Samples
8
Type
Expression profiling by high...
Submitted
2019-05-25

In response to various stimuli, vascular smooth muscle cells (SMCs) can de-differentiate, proliferate and migrate in a process known as phenotypic modulation. However, the phenotype of modulated SMCs in vivo during atherosclerosis and the influence of this process on coronary artery disease (CAD) risk have not been clearly established. Using single cell RNA sequencing, we comprehensively characterized the transcriptomic phenotype of modulated SMCs in vivo in atherosclerotic lesions of both mouse...

Provenance — who produced it, who reused it

Linked to 18 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Robert C WirkaDhananjay WaghDavid T PaikMilos PjanicTrieu NguyenClint L MillerRamen KunduManabu NagaoJohn CollerTiffany KoyanoRobyn FongY J WooBoxiang LiuStephen B MontgomeryJoseph C WuKuixi ZhuRui ChangMelissa AlampreseMichelle D TallquistJuyong B KimThomas Quertermous
Reused by

16 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Excellent human bulk RNA-seq with perfect base quality (97.2% Q30, Phred 38.8) enabling highly accurate mapping. Short reads (26bp) and moderate duplication (21.67%) limit alignment breadth and may bias expression quantification, but base accuracy is outstanding for reuse.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.011 measured
pct q20 bases 99.2 measured
pct q30 bases 97.2 measured
gc content pct 48.4 measured
mean read length 26 measured
mean base quality 38.8 measured
adapter content pct 0 measured
duplication rate pct 21.67 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.2 measured ×1 100%
mean base quality 38.8 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 21.67 measured ×0.4 100%
QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it mean score 70