In vivo RNA-sequencing of Vibrio cholerae during intestinal colonization of infant rabbits
To understand how cholera toxin (CT) produced by Vibrio cholerae modulates gene expression of this organism within the intestine, RNA-seq analysis was performed on two samples each of WT and the ∆ctx mutant bacteria harvested from either the infant rabbit ileum or the cecum one-day post-intragastric infection. We found that 243 genes that were significantly up-regulated in the WT compared to the ∆ctx mutant and these included 101 genes in ileum samples, 118 in the cecum samples, and 24 in both s...
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Cholera toxin promotes pathogen acquisition of host-derived nutr... 2019 · 130 cites
Deep data QC
83/100 · BStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
This Vibrio cholerae RNA-Seq with unavailable details shows high quality (96.5% Q30) and mean read length of 100 bp. Metadata unavailability prevents platform identification.
The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0