Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE134240

GEO first seen 2019

Single Cell RNA-seq analysis of gene expression patterns of cells from stem-cell-derived-synthetic-blastocysts, called as 'ES-' or 'EPS-blastoids'.

Organism
Mus musculus
Samples
1
Type
Expression profiling by high...
Submitted
2019-07-13

To test the efficacy of stem cell lines to generate 'stem-cell-derived-synthetic blastocysts', we dissociated ES- (built by conventional embryonic stem cells and trophoblast stem cells) or EPS-blastoids (built by extended potential pluripotent stem cells and trophoblast stem cells) into single cells following 96h of culture for single-cell transcriptome analysis.

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Joachim De JongheBerna SozenAndy CoxFlorian HollfelderMagdalena Zernicka-Goetz
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

91/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.002 measured
pct q20 bases 93 measured
pct q30 bases 90.7 measured
gc content pct 42.5 measured
mean read length 61 measured
mean base quality 33.6 measured
adapter content pct 1.19 measured
duplication rate pct 48.98 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 91/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.7 measured ×1 100%
mean base quality 33.6 measured ×0.6 93%
adapter content pct 1.19 measured ×0.4 100%
duplication rate pct 48.98 measured ×0.4 58%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0