Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE135140

GEO first seen 2019

U1 snRNP complex with cleavage and polyadenylation factors controls mRNA transcription termination

Organism
Homo sapiens
Samples
22
Type
Expression profiling by high...
Submitted
2019-07-30

Full-length transcription in the majority of human genes depends on U1 snRNP (U1) to co-transcriptionally suppress transcription-terminating premature 3’-end cleavage and polyadenylation (PCPA) from cryptic polyadenylation signals (PASs) in introns. However, the mechanism of this U1 activity, termed telescripting, is unknown. Here, we captured a complex, comprising U1 and CPA factors (U1–CPAFs), that binds intronic PASs and suppresses PCPA. U1–CPAFs are distinct from U1-spliceosomal complexes; t...

Provenance — who produced it, who reused it

Linked to 4 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

72/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
22 / 22 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 723476189900 reported
total reads 2570472833 reported
n content pct 0.001 measured
pct q20 bases 97.8 measured
pct q30 bases 94.4 measured
gc content pct 55.1 measured
mean read length 150 measured
mean base quality 36 measured
adapter content pct 27.43 measured
duplication rate pct 60.64 measured
supplementary file types BW reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 72/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.4 measured ×1 100%
mean base quality 36 measured ×0.6 100%
adapter content pct 27.43 measured ×0.4 0%
duplication rate pct 60.64 measured ×0.4 32%
QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0