Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE135202

GEO first seen 2019

Tracing the first hematopoietic stem cell generation in human embryo by single-cell RNA sequencing

Organism
Homo sapiens
Samples
7
Type
Expression profiling by high...
Submitted
2019-07-31

Tracing the emergence of the first hematopoietic stem cells (HSCs) in human embryos, particularly the scarce and transient precursors thereof, is so far challenging, largely due to technical limitations and material rarity. Here, using single-cell RNA sequencing, we constructed the first genome-scale gene expression landscape covering the entire course of endothelial-to-HSC transition during human embryogenesis. The transcriptomically defined HSC-primed hemogenic endothelial cells (ECs) were cap...

Provenance — who produced it, who reused it

Linked to 6 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Yang ZengJian HeZhijie BaiZongcheng LiYu LanBing Liu
Reused by

3 further papers cite this accession but reuse could not be confirmed.

Deep data QC

39/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.005 measured
pct q20 bases 75 measured
pct q30 bases 57.5 measured
gc content pct 33.1 measured
mean read length 150 measured
mean base quality 29.4 measured
adapter content pct 0.11 measured
duplication rate pct 7.83 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 39/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 57.5 measured ×1 0%
mean base quality 29.4 measured ×0.6 23%
adapter content pct 0.11 measured ×0.4 100%
duplication rate pct 7.83 measured ×0.4 100%
QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0