Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE135710

GEO first seen 2021

Single-cell RNA-seq of B cell polustion in breast cancer patients

Organism
Homo sapiens; Mus musculus
Samples
4
Type
Expression profiling by high...
Submitted
2019-08-12

Re-education of immune cells in tumor microenvironment is required for optimal treatment outcome. The prevailing notion is that B cells, a major immune cell type in tumors, are immunosuppressive and pro-tumoral. However, why B cells inhibit tumor progression in certain contexts remains unexplained. By single cell dissection of B cell heterogeneity in longitudinal samples of breast cancer patients before and after neoadjuvant chemotherapy, we revealed a distinct subset of B cells emerges after ch...

Provenance — who produced it, who reused it

Linked to 4 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Yiwen LuQiyi ZhaoJian-You LiaoErwei SongQidong XiaJiaqian LiBoxuan ZhouYingying YeCan DiShubin YuJunchao CaiShicheng Su
Reused by

3 further papers cite this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.001 measured
pct q20 bases 69.6 measured
pct q30 bases 52.5 measured
gc content pct 35.5 measured
mean read length 150 measured
mean base quality 27.9 measured
adapter content pct 1.25 measured
duplication rate pct 14.28 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 52.5 measured ×1 0%
mean base quality 27.9 measured ×0.6 0%
adapter content pct 1.25 measured ×0.4 100%
duplication rate pct 14.28 measured ×0.4 100%
QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0