Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE135922

GEO first seen 2020

Single-cell transcriptomics of the human retinal pigment epithelium and choroid in health and macular degeneration

Organism
Homo sapiens
Samples
14
Type
Expression profiling by high...
Submitted
2019-08-17

Purpose: The goals of this study were to identify cell specific expression patterns of the retinal pigment epithelium (RPE) and a variety of choroidal cell types originating from the macula and the periphery of human donor eyes, with a particular emphasis on identifying expression signatures of choroidal endothelial cells. Methods: Independent libraries were prepared for macular and peripheral samples of combined RPE/choroid from seven donors in two single cell sequencing experiments. In the fir...

Provenance — who produced it, who reused it

Linked to 10 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Todd E ScheetzRobert F Mullins
Reused by

9 further papers cite this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
176 / 14 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 729088706914 reported
total reads 4258180745 reported
n content pct 0.001 measured
pct q20 bases 61.6 measured
pct q30 bases 46.8 measured
gc content pct 33.5 measured
mean read length 151 measured
mean base quality 26.3 measured
adapter content pct 0.3 measured
duplication rate pct 7.26 measured
supplementary file types TSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 46.8 measured ×1 0%
mean base quality 26.3 measured ×0.6 0%
adapter content pct 0.3 measured ×0.4 100%
duplication rate pct 7.26 measured ×0.4 100%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0