Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE136103

GEO first seen 2020

Resolving the fibrotic niche of human liver cirrhosis using single-cell transcriptomics

Organism
Homo sapiens; Mus musculus
Samples
26
Type
Expression profiling by high...
Submitted
2019-08-21

We profile the transcriptomes of over 100,000 human single cells, yielding molecular definitions for non-parenchymal cell types present in healthy and cirrhotic human liver. We uncover a novel scar-associated TREM2+CD9+ macrophage subpopulation, which expands in liver fibrosis, differentiates from circulating monocytes, has a corollary population in mouse liver fibrosis and is pro-fibrogenic. We also define novel ACKR1+ and PLVAP+ endothelial cells which expand in cirrhosis, are topographically...

Provenance — who produced it, who reused it

Linked to 27 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Prakash RamachandranNeil C HendersonJohn R Wilson-Kanamori
Reused by

24 further papers cite this accession but reuse could not be confirmed.

Deep data QC

60/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens; Mus musculus
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
52 / 26 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 751599612145 reported
total reads 9119426267 reported
n content pct 0.007 measured
pct q20 bases 88.4 measured
pct q30 bases 72.8 measured
gc content pct 46.9 measured
mean read length 75 measured
mean base quality 33.1 measured
adapter content pct 0 measured
duplication rate pct 28.3 measured
supplementary file types MTX, TSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 60/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 72.8 measured ×1 14%
mean base quality 33.1 measured ×0.6 85%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 28.3 measured ×0.4 100%
QC cost 6 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0