Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE137396

GEO first seen 2021

Characterization of transcriptional analysis of LKB1 mutant lung nodules

Organism
Mus musculus
Samples
10
Type
Expression profiling by high...
Submitted
2019-09-13

Inactivating mutations in LKB1/STK11 are present in ~20% of non-small cell lung cancers (NSCLC) and portend poor response to anti-PD-1 immunotherapy in patients and genetically engineered mouse model (GEMMs). Here, we sought to uncover the basis for immunotherapy resistance of these tumors and to define strategies that overcome this barrier. Whereas high tumor mutational burden (TMB) often correlates with response to anti-PD1 treatment, we found that LKB1-deficient NSCLCs from non-smokers and GE...

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Charles M PerouKwok-Kin Wong
Reused by

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
10 / 10 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 65935003000 reported
total reads 659350030 reported
n content pct 0.003 measured
pct q20 bases 98.3 measured
pct q30 bases 95.8 measured
gc content pct 49.3 measured
mean read length 50 measured
mean base quality 35.9 measured
adapter content pct 0.01 measured
duplication rate pct 29.2 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.8 measured ×1 100%
mean base quality 35.9 measured ×0.6 100%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 29.2 measured ×0.4 100%
QC cost 20 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0