Genome-Wide Analysis In Vivo of Translation with Nucleotide Resolution Using Ribosome Profiling
Techniques for systematically monitoring protein translation have lagged far behind methods for measuring mRNA levels. Here we present a ribosome profiling strategy, based on deep sequencing of ribosome protected mRNA fragments, that enables genome-wide investigation of translation with sub-codon resolution. We used this technique to monitor translation in budding yeast under both rich and starvation conditions. These studies defined the protein sequences being translated and found extensive tra...
Provenance — who produced it, who reused it
Linked to 17 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Rate-Limiting Steps in Yeast Protein Translation 2013 · 530 cites
- Balanced Codon Usage Optimizes Eukaryotic Translational Efficien... 2012 · 327 cites
- Positively Charged Residues Are the Major Determinants of Riboso... 2013 · 316 cites
- The effect of tRNA levels on decoding times of mRNA codons 2014 · 288 cites
- Accounting for biases in riboprofiling data indicates a major ro... 2014 · 154 cites
- Codon-by-Codon Modulation of Translational Speed and Accuracy Vi... 2014 · 132 cites
- Simultaneous mapping of transcript ends at single-nucleotide res... 2014 · 116 cites
- riboCIRC: a comprehensive database of translatable circRNAs 2021 · 82 cites
- The deca-GX3 proteins Yae1-Lto1 function as adaptors recruiting... 2015 · 72 cites
8 further papers cite this accession but reuse could not be confirmed.
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
No quantitative QC rubric exists for this data type yet, so it is deliberately left unscored — this is an honest "not applicable", not a poor rating.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently