Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE140203

GEO first seen 2020

Integrative single-cell chromatin and transcriptome profiling uncovers cell-type specific regulatory interactions

Organism
Homo sapiens; Mus musculus
Samples
20
Type
Expression profiling by high...
Submitted
2019-11-11

Simultaneous measurement of different molecular aspects of gene regulation can help infer functional relationships between genome regulation and gene expression, and understand their distinct and contribution to cell phenotype. Here, we present SHARE-seq, a highly scalable, sensitive, and cost-effective approach for joint measurement of chromatin accessibility and gene expression from the same single cells. Applying SHARE-seq to adult mouse tissues (skin, brain, lung) we show a direct congruence...

Provenance — who produced it, who reused it

Linked to 21 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Sai MaJason Buenrostro
Reused by

17 further papers cite this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ATAC-seq
Organism
Homo sapiens; Mus musculus
Instrument
Illumina NovaSeq 6000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), BAM/CRAM (aligned)
N numbers (samples, groups)
20 / 20 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 192198520503 reported
total reads 2387427195 reported
n content pct 0.002 measured
pct q20 bases 99.3 measured
pct q30 bases 99.1 measured
gc content pct 43.6 measured
mean read length 29.4 measured
mean base quality 35.1 measured
adapter content pct 0.01 measured
duplication rate pct 42.95 measured
supplementary file types BED, TXT reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ATAC-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 99.1 measured ×1 100%
QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0