Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE140262

GEO first seen 2020

Solo: doublet identification via semi-supervised deep learning

Organism
Mus musculus
Samples
4
Type
Expression profiling by high...
Submitted
2019-11-12

We developed a semi-supervised deep learning framework for the identification of doublets in scRNA-seq analysis called Solo. To validate our method, we used MULTI-seq, cholesterol modified oligos (CMOs), to experimentally identify doublets in a solid tissue with diverse cell types, mouse kidney, and showed Solo recapitulated experimentally identified doublets.

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Nicholas BernsteinNicole FongMargaret RoyIrene LamDavid HendricksonDavid Kelley
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

26/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.019 measured
pct q20 bases 59.4 measured
pct q30 bases 41.9 measured
gc content pct 22.4 measured
mean read length 91 measured
mean base quality 25 measured
adapter content pct 0.25 measured
duplication rate pct 48.66 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 26/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 41.9 measured ×1 0%
mean base quality 25 measured ×0.6 0%
adapter content pct 0.25 measured ×0.4 100%
duplication rate pct 48.66 measured ×0.4 59%
QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0