Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE142025

GEO first seen 2021

Comparison of Kidney Transcriptomic Profiles of Early and Advanced Diabetic Nephropathy Reveals Potential New Mechanisms for Disease Progression

Organism
Homo sapiens
Samples
36
Type
Expression profiling by high...
Submitted
2019-12-13

To identify the factors mediating the progression of di- abetic nephropathy (DN), we performed RNA sequencing of kidney biopsy samples from patients with early DN, advanced DN, and normal kidney tissue from nephrectomy samples. A set of genes that were upregulated at early but downregulated in late DN were shown to be largely renoprotective, which included genes in the retinoic acid pathway and glucagon-like peptide 1 receptor. Another group of genes that were downregulated at early but highly u...

Provenance — who produced it, who reused it

Linked to 26 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Ying FanZhengzi YiWeijia ZhangKyung LeeJohn C He
Reused by

23 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
36 / 36 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 699093542400 reported
total reads 3495467712 reported
n content pct 0.005 measured
pct q20 bases 98.6 measured
pct q30 bases 96 measured
gc content pct 51.3 measured
mean read length 100 measured
mean base quality 39.4 measured
adapter content pct 0.29 measured
duplication rate pct 25.32 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96 measured ×1 100%
mean base quality 39.4 measured ×0.6 100%
adapter content pct 0.29 measured ×0.4 100%
duplication rate pct 25.32 measured ×0.4 100%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0