Co-transcriptional splicing regulates 3' end cleavage during mammalian erythropoiesis
Pre-mRNA processing steps are tightly coordinated with transcription in many organisms. To determine how co-transcriptional splicing is integrated with transcription elongation and 3' end formation in mammalian cells, we performed long read sequencing of individual nascent RNAs and PRO-seq during mouse erythropoiesis. Splicing was not accompanied by transcriptional pausing and was detected when RNA polymerase II (Pol II) was within 75-300 nucleotides of 3' splice sites (3'SSs), often during tran...
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deep data QC
83/100 · BStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
This mouse RNA-Seq with unavailable details shows perfect quality (98.9% Q30) but with an extremely unusual mean read length of 858.4 bp, suggestive of potential data entry error. Metadata unavailability prevents verification.
The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0