Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE145363

GEO first seen 2020

Targeting FTO suppresses cancer stem cell maintance and immune evasion

Organism
Mus musculus
Samples
4
Type
Expression profiling by high...
Submitted
2020-02-16

We conducted RNA sequencing with poly A RNA isolated from spleen mononuclear cells of MLL-AF9 (MA9) mouse models after treatment with PBS and FTO inhibitor CS1.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Jianjun ChenRui SuLei Dong

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This mouse RNA-Seq with unavailable metadata shows good quality (94.9% Q30) and mean read length of 50.8 bp. Missing details limit assessment.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.001 measured
pct q20 bases 97.6 measured
pct q30 bases 94.9 measured
gc content pct 48.7 measured
mean read length 50.8 measured
mean base quality 35.6 measured
adapter content pct 0.16 measured
duplication rate pct 21.48 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.9 measured ×1 100%
mean base quality 35.6 measured ×0.6 100%
adapter content pct 0.16 measured ×0.4 100%
duplication rate pct 21.48 measured ×0.4 100%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0