Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

GSE147405

GEO first seen 2021

Context specificity of the EMT transcriptional response

Organism
Homo sapiens
Samples
10
Type
Expression profiling by high...
Submitted
2020-03-23

Epithelial-mesenchymal plasticity contributes to a variety of biological processes, including tumour progression. A variety of epithelial-mesenchymal transition (EMT) responses have been reported and no common, EMT-defining gene expression program has been identified. Here, we have performed a comparative analysis of the EMT response, leveraging highly multiplexed single-cell RNA sequencing (scRNA-seq) to measure expression profiles of 103,999 cells from 960 samples, comprising 12 EMT time cours...

Provenance — who produced it, who reused it

Linked to 13 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
David P CookBarbara C Vanderhyden
Reused by

10 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq from Homo sapiens; perfect A-grade (100/100). Exceptionally low duplication (1.46%) and high base quality (95.3% Q30, Q34.2 mean) are the dual drivers; despite short 25.6bp reads, minimal duplication risk and reliable base calls make this ideal for precise differential-expression reuse and meta-analysis pooling.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.001 measured
pct q20 bases 96.9 measured
pct q30 bases 95.3 measured
gc content pct 47.4 measured
mean read length 25.6 measured
mean base quality 34.2 measured
adapter content pct 0 measured
duplication rate pct 1.46 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.3 measured ×1 100%
mean base quality 34.2 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 1.46 measured ×0.4 100%
QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 flagged mean score 53