Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE150316

GEO first seen 2020

Spectrum of Viral Load and Host Response Seen in Autopsies of SARS-CoV-2 Infected Lungs

Organism
Homo sapiens
Samples
88
Type
Expression profiling by high...
Submitted
2020-05-11

The relationship of SARS-CoV-2 lung infection and severity of pulmonary disease is not fully understood. We visualized viral RNA by in situ hybridization and assessed the immune infiltrate using immunohistochemistry and total RNA sequencing on five COVID-19 positive patients. Patients with high levels of viral RNA showed hyaline membranes on histology, extensive pneumocyte loss, low T-cell numbers and were enriched for the interferon gene signature, while patients with lower levels of viral RNA...

Provenance — who produced it, who reused it

Linked to 23 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Niyati DesaiAzfar NeyazAnnamaria SzabolcsAngela ShihLinda T NiemanVishal ThaparAlexander SolovyovArnav MehtaDavid LiebAnupriya S KulkarniChristopher JaicksJonathan ChenChristopher J PintoRobert MonroeDejan JuricIvan ChebibNir HacohenBenjamin D GreenbaumMiguel N RiveraJames R StoneDavid T TingIkram Deshpande
Reused by

21 further papers cite this accession but reuse could not be confirmed.

Deep data QC

99/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
88 / 88 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 96815311822 reported
total reads 663796968 reported
n content pct 0.433 measured
pct q20 bases 96.2 measured
pct q30 bases 94.9 measured
gc content pct 48.7 measured
mean read length 74.6 measured
mean base quality 34.5 measured
adapter content pct 0.01 measured
duplication rate pct 32.82 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 99/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.9 measured ×1 100%
mean base quality 34.5 measured ×0.6 100%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 32.82 measured ×0.4 94%
QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0