GSE153521

GEO

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

82/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human RNA-seq via Illumina HiSeq 4000 with excellent quality (99.3% Q20, 99.3% Q30) but relatively short reads (76 bp), enabling high-confidence gene expression quantification and isoform detection. Short read length may complicate mapping and isoform assembly compared to longer-read platforms.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Instrument

Illumina HiSeq 4000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

8 / 8 runs

Completeness

100%

Metrics (value · how obtained)

checksum ok yes reported

total bases 39821278288 reported

total reads 523964188 reported

n content pct 0.004 measured

pct q20 bases 99.3 measured

pct q30 bases 99.3 measured

gc content pct 51.9 measured

mean read length 76 measured

mean base quality 29.8 measured

adapter content pct 0.07 measured

duplication rate pct 25.63 measured

supplementary file types TXT reported

How this grade was computed

Weighted mean of 4 scored metric(s) → 82/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 99.3 measured ×1 100%

mean base quality 29.8 measured ×0.6 30%

adapter content pct 0.07 measured ×0.4 100%

duplication rate pct 25.63 measured ×0.4 100%

QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0