Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE154778

GEO first seen 2023

Single-cell transcriptomics analysis of pancreatic primary tumor and metastatic biopsy tissues

Organism
Homo sapiens
Samples
16
Type
Expression profiling by high...
Submitted
2020-07-20

The single-cell RNA profiles of dissociated 10 pancreatic primary tumors and 6 metastatic biopsies were obtained using the 10x Genomics Chromium platform

Provenance — who produced it, who reused it

Linked to 19 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Wei LinPawan NoelHaiyong Han
Reused by

16 further papers cite this accession but reuse could not be confirmed.

Deep data QC

94/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
120 / 16 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 215682469474 reported
total reads 2033915483 reported
n content pct 0.001 measured
pct q20 bases 96.1 measured
pct q30 bases 93 measured
gc content pct 45.2 measured
mean read length 109 measured
mean base quality 37 measured
adapter content pct 0.03 measured
duplication rate pct 47.38 measured
supplementary file types CSV, MTX, TSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 94/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93 measured ×1 100%
mean base quality 37 measured ×0.6 100%
adapter content pct 0.03 measured ×0.4 100%
duplication rate pct 47.38 measured ×0.4 61%
QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0