Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

GSE154826

GEO first seen 2022

CITEseq analysis of non-small-cell lung cancer lesions

Organism
Homo sapiens
Samples
166
Type
Expression profiling by high...
Submitted
2020-07-21

Immunotherapy is becoming a mainstay in the treatment of NSCLC. We profiled immune cells of 35 early stage NSCLC lesions using multiscale single cell sequencing, including scRNAseq, CITEseq, and TCRseq.

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Andrew M LeaderJohn A GroutChristie ChangBarbara MaierAlexandra TabachnikovaLaura WalkerAlona LanskyJessica LeBerichelNaussica MalissenMelanie DavilaJerome MartinGiuliana MagriKevin TuballesZhen ZhaoFrancesca PetraliaRobert SamsteinNatalie Roy D'AmoreGavin ThurstonAlice KamphorstAndrea WolfRaja FloresPei WangMary B BeasleyHelene SalmonAdeeb H RahmanThomas U MarronEphraim KenigsbergMiriam Merad
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

53/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Human bulk-RNA-seq with poor base quality (mean 26.7, scored 0/100) and extreme PCR duplication (79.87%, scored 0/100), making the dataset unsuitable for reliable quantification of gene expression due to high noise and artificial abundance inflation.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Files available
CSV
Metrics (value · how obtained)
n content pct 0.197 measured
pct q20 bases 87.6 measured
pct q30 bases 87.6 measured
gc content pct 10.9 measured
mean read length 55 measured
mean base quality 26.7 measured
adapter content pct 0 measured
duplication rate pct 79.87 measured
supplementary file types CSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 53/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 87.6 measured ×1 88%
mean base quality 26.7 measured ×0.6 0%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 79.87 measured ×0.4 0%

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 96