Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE155673

GEO first seen 2021

Systems biological assessment of immunity to severe and mild COVID-19 infections

Organism
Homo sapiens
Samples
24
Type
Expression profiling by high...
Submitted
2020-08-04

CITE-seq of 7 patients with COVID-19 and 5 healthy controls

Provenance — who produced it, who reused it

Linked to 6 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Madeleine K ScottPrabhu S ArunachalamFlorian Wimmers
Reused by

5 further papers cite this accession but reuse could not be confirmed.

Deep data QC

75/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Human RNA-seq with 91 bp reads at 90.2% Q20 and 78.1% Q30 exhibits declining base quality across the read, particularly pronounced in later cycles. The 47.6% GC and mean quality 34.5 support basic transcript counting; however, the low Q30 percentage warrants conservative mapping parameters and post-alignment quality filtering to avoid accumulating substitution errors.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Files available
CSV, MTX, TSV
Metrics (value · how obtained)
n content pct 0.003 measured
pct q20 bases 90.2 measured
pct q30 bases 78.1 measured
gc content pct 47.6 measured
mean read length 91 measured
mean base quality 34.5 measured
adapter content pct 0.03 measured
duplication rate pct 29.42 measured
supplementary file types CSV, MTX, TSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 75/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.1 measured ×1 41%
mean base quality 34.5 measured ×0.6 100%
adapter content pct 0.03 measured ×0.4 100%
duplication rate pct 29.42 measured ×0.4 100%

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 95