Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE156210

GEO first seen 2020

Cholesterol pathway inhibition induces TGFβ signaling to promote basal differentiation in pancreatic cancer

Organism
Mus musculus
Samples
5
Type
Expression profiling by high...
Submitted
2020-08-13

Oncogenic transformation alters the metabolism of cellular lipids to sustain tumor growth. We define a reciprocal mechanism by which cholesterol metabolism controls the formation and differentiation of pancreatic ductal adenocarcinoma (PDAC). Disruption of distal cholesterol biosynthesis by conditional inactivation of Nsdhl, or treatment with cholesterol-lowering statins caused murine pancreatic carcinomas induced by KrasG12D expression and homozygous Trp53 loss to undergo a differentiation...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Igor AstsaturovSuraj Peri

Deep data QC

51/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Mouse 150 bp paired-end RNA-seq with moderate quality metrics (Q30: 68.6%, mean quality 32.3), representing lower-tier sequencing reliability common in older or cost-optimized studies. The 36% GC content is notably depressed, potentially indicating GC-bias or sample-specific transcript composition. Search terms: mouse bulk RNA-seq, paired-end transcriptomics.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.005 measured
pct q20 bases 83.4 measured
pct q30 bases 68.6 measured
gc content pct 36 measured
mean read length 150 measured
mean base quality 32.3 measured
adapter content pct 2.64 measured
duplication rate pct 10.02 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 51/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 68.6 measured ×1 0%
mean base quality 32.3 measured ×0.6 72%
adapter content pct 2.64 measured ×0.4 96%
duplication rate pct 10.02 measured ×0.4 100%
QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0