Single-cell RNA-sequencing of iBlastoids generated by reprogramming
Here, we report an in vitro model of the human blastocyst by reprogramming fibroblasts, termed iBlastoids. Comprehensive characterization of these iBlastoids demonstrated that they can accurately represent the overall architecture of human blastocysts including an inner cell mass-like structure, marked by classic EPI-associated genes surrounded by an outer layer of cells with key molecular characteristics of the TE and a cavity resembling the blastocoel. Single-cell transcriptomics confirmed the...
Provenance — who produced it, who reused it
Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Naive stem cell blastocyst model captures human embryo lineage s... 2021 · 317 cites
- Modeling human extraembryonic mesoderm cells using naive pluripo... 2022 · 86 cites
1 further paper cites this accession but reuse could not be confirmed.
Deep data QC
93/100 · AStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Bulk RNA-seq with excellent base quality (Q30=93.2%, mean_BQ=35.8) limited only by moderate duplication (47.86%); despite a fetch error flag, measured metrics show strong overall quality; good read length (94bp) and high Q30 make this dataset highly suitable for reuse.
The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0
Scientific quality
Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.