Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE156963

GEO first seen 2020

To determine the differentially expressed genes in cancer associated fibroblasts upon ablation of the glutamatergic synapse protein NetrinG1. [RNA-Seq]

Organism
Homo sapiens
Samples
8
Type
Expression profiling by high...
Submitted
2020-08-27

Purpose: To determine the differentially expressed genes in cancer associated fibroblasts upon ablation of the glutamatergic synapse protein NetrinG1. Methods: Cancer associated fibroblast mRNA profiles were generated from Control and NetrinG1 KO fibroblast lines, with controls done in duplicate and KOs in triplicate. Results: Comparing the mRNA profiles of CON and NetrinG1 KO CAFs, we observed a reversion of many pro-tumor genes and associated pathways upon ablation of NetrinG1 in CAFs. Conclus...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Edna CukiermanRalph Francescone

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This human RNA-Seq with unavailable metadata shows very high quality (97.1% Q30) and mean read length of 54 bp. Missing details prevent characterization.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0 measured
pct q20 bases 98.5 measured
pct q30 bases 97.1 measured
gc content pct 49.7 measured
mean read length 54 measured
mean base quality 38.4 measured
adapter content pct 0 measured
duplication rate pct 29.88 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.1 measured ×1 100%
mean base quality 38.4 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 29.88 measured ×0.4 100%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0