Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE159249

GEO

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

87/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq with excellent base quality (Q30=89.4%, mean_BQ=35.3) severely compromised by 61% sequence duplication, indicating heavy PCR amplification bias; this extreme duplication substantially reduces effective library complexity and statistical power for expression quantification.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
12 / 12 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 62700333100 reported
total reads 627003331 reported
n content pct 0.173 measured
pct q20 bases 94.3 measured
pct q30 bases 89.4 measured
gc content pct 49.8 measured
mean read length 50 measured
mean base quality 35.3 measured
adapter content pct 0 measured
duplication rate pct 60.99 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 87/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 89.4 measured ×1 97%
mean base quality 35.3 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 60.99 measured ×0.4 31%
QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0