Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE162855

GEO first seen 2021

High-Throughput Transcriptomics Platform for Screening Environmental Chemicals

Organism
Homo sapiens
Samples
1,134
Type
Expression profiling by high...
Submitted
2020-12-08

New approach methodologies (NAMs) that efficiently provide information about chemical hazard without using whole animals are needed to accelerate the pace of chemical safety assessments. Technological advancements in gene expression assays have made in vitro high-throughput transcriptomics (HTTr) a feasible option for NAMs-based hazard characterization of environmental chemicals. In the present study, we evaluated the Templated Oligo with Sequencing Readout (TempO-Seq) assay for HTTr concentrati...

Provenance — who produced it, who reused it

Linked to 4 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Joshua A HarrillLogan J EverettDerik E HaggardThomas SheffieldJoseph BundyClinton M WillisRussell S ThomasImran ShahRichard S Judson
Reused by

3 further papers cite this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.013 measured
pct q20 bases 97.9 measured
pct q30 bases 95.7 measured
gc content pct 51.8 measured
mean read length 50 measured
mean base quality 36.7 measured
adapter content pct 0 measured
duplication rate pct 88.49 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.7 measured ×1 100%
mean base quality 36.7 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 88.49 measured ×0.4 0%
QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0