Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE163804

GEO first seen 2021

A PP2A-Integrator complex fine-tunes transcription by opposing CDK9 [ChIP-seq]

Organism
Drosophila melanogaster; Homo sapiens
Samples
33
Type
Genome binding/occupancy pro...
Submitted
2020-12-24

Gene expression by RNA Polymerase II (RNAPII) is tightly controlled by Cyclin-dependent kinases (CDKs) at discrete checkpoints during the transcription cycle. The RNAPII pausing checkpoint, engaged after transcription initiation, is controlled by CDK9 to regulate transcription in metazoans. We discovered that CDK9-mediated RNAPII pause-release is functionally opposed by a protein phosphatase 2A (PP2A) complex. PP2A dynamically competes for key CDK9 substrates, DSIF and RNAPII-CTD, and is recrui...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Stephin J VervoortIzabela TodorovskiMatteo CostacurtaJennifer R DevlinRicky W Johnstone

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.001 measured
pct q20 bases 91.2 measured
pct q30 bases 88.1 measured
gc content pct 40.4 measured
mean read length 75 measured
mean base quality 33.1 measured
adapter content pct 6.55 measured
duplication rate pct 0.94 measured
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.1 measured ×1 91%
QC cost 45 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0