Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE166692

GEO first seen 2023

Sci-Space E14 Mouse Embryo Data

Organism
Mus musculus
Samples
960
Type
Expression profiling by high...
Submitted
2021-02-12

Spatial patterns of gene expression span many scales, and are shaped by both local (e.g. cell-cell interactions) and global (e.g. tissue, organ) context. However, most in situ methods for profiling gene expression either average local contexts or are restricted to limited fields of view. Here we introduce sci-Space, a scale-flexible method that retains single cell resolution while resolving spatial heterogeneity in gene expression at larger scales. As a proof-of-concept, we apply sci-Space to th...

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Sanjay R SrivatsanMary Regier
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

93/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
NextSeq 550
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
959 / 960 runs
Completeness
99.9%
Metrics (value · how obtained)
checksum ok yes reported
total bases 275332933540 reported
total reads 3933327622 reported
n content pct 0.002 measured
pct q20 bases 97.4 measured
pct q30 bases 96.2 measured
gc content pct 47.2 measured
mean read length 18 measured
mean base quality 34 measured
adapter content pct 0 measured
duplication rate pct 48.19 measured
supplementary file types MTX, RDS, TSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 93/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.2 measured ×1 100%
mean base quality 34 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 48.19 measured ×0.4 60%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0