Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE171110

GEO first seen 2023

Whole blood transciptome analysis in severe Covid-19 patients and healthy donors

Organism
Homo sapiens
Samples
54
Type
Expression profiling by high...
Submitted
2021-03-29

The identification of COVID-19 patients with high-risk of severe disease is a challenge in routine care. We performed blood RNA-seq gene expression analyses in severe hospitalized patients compared to healthy donors. Supervised and unsupervised analyses revealed a high abundance of CD177, a specific neutrophil activation marker, contributing to the clustering of severe patients. Gene abundance correlated with high serum levels of CD177 in severe patients. These results highlight neutrophil activ...

Provenance — who produced it, who reused it

Linked to 34 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Yves LévyAurélie WiedemannBoris P HejblumMélany DurandCécile LefebvreMathieu SurénaudChristine LacabaratzMatthieu PerreauEmile FoucatMarie DéchenaudPascaline TisserandFabiola BlengioBenjamin HivertMarine GauthierMinerva Cervantes-GonzalezDelphine BacheletCédric LaouénanLila BouadmaJean-François TimsitYazdan YazdanpanahGiuseppe PantaleoHakim HociniRodolphe Thiébaut
Reused by

33 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
432 / 54 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 235905944531 reported
total reads 2354282209 reported
n content pct 0.001 measured
pct q20 bases 95.3 measured
pct q30 bases 90.2 measured
gc content pct 48.3 measured
mean read length 100.3 measured
mean base quality 34.9 measured
adapter content pct 0 measured
duplication rate pct 20.09 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.2 measured ×1 100%
mean base quality 34.9 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 20.09 measured ×0.4 100%
QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0