Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

GSE184880

GEO first seen 2022

Single-cell RNA sequencing reveals tissue architecture during human high-grade serous ovarian cancer progression

Organism
Homo sapiens
Samples
12
Type
Expression profiling by high...
Submitted
2021-09-27

As the most fatal type of gynecological cancers, high-grade serous ovarian cancer (HGSOC) exhibits heterogeneity that obstacles therapeutics. Up to date, the pathogenesis of HGSOC remains poorly understood. Here, we performed the deep single-cell RNA sequencing (scRNA-seq) using 59,324 cells isolated from seven treatment-naïve HGSOC patients at early or late tumor stages and five age-matched non-malignant ovarian samples. Our sequencing data showed a highly complex ecosystem of tumor, immune an...

Provenance — who produced it, who reused it

Linked to 20 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

19 further papers cite this accession but reuse could not be confirmed.

Deep data QC

38/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina NovaSeq 6000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
60 / 12 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 1609678704300 reported
total reads 5365595681 reported
n content pct 0 measured
pct q20 bases 76.8 measured
pct q30 bases 60.5 measured
gc content pct 30.5 measured
mean read length 150 measured
mean base quality 29 measured
adapter content pct 0.04 measured
duplication rate pct 5.3 measured
supplementary file types MTX, TSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 38/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 60.5 measured ×1 0%
mean base quality 29 measured ×0.6 17%
adapter content pct 0.04 measured ×0.4 100%
duplication rate pct 5.3 measured ×0.4 100%
QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0