Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE19480

GEO first seen 2013

Understaning mechanisms underlying human gene expression variation with RNA sequencing

Organism
Homo sapiens
Samples
161
Type
Expression profiling by high...
Submitted
2009-12-15

Understanding the genetic mechanisms underlying natural variation in gene expression is a central goal of both medical and evolutionary genetics, and studies of expression quantitative trait loci (eQTLs) have become an important tool for achieving this goal. While all eQTL studies to date have assayed mRNA levels using expression microarrays, recent advances in RNA sequencing enable the analysis of transcript variation at unprecedented resolution. We sequenced RNA from 69 lymphoblastoid cell lin...

Provenance — who produced it, who reused it

Linked to 16 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Joseph PickrellJonathan PritchardYoav Gilad
Reused by

10 further papers cite this accession but reuse could not be confirmed.

Deep data QC

35/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.006 measured
pct q20 bases 90.6 measured
pct q30 bases 58.7 measured
gc content pct 51.1 measured
mean read length 35 measured
mean base quality 28.3 measured
adapter content pct 0 measured
duplication rate pct 19.02 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 35/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 58.7 measured ×1 0%
mean base quality 28.3 measured ×0.6 5%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 19.02 measured ×0.4 100%
QC cost 40 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0