Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE20748

GEO first seen 2012

DNA Methylation Mediated by a MicroRNA Pathway

Organism
Oryza sativa
Samples
7
Type
Non-coding RNA profiling by...
Submitted
2010-03-11

In plants, the known microRNAs (miRNAs) are produced as ~21 nucleotide (nt) duplexes from their precursors by Dicer like 1 (DCL1). They are incorporated into Argonaute 1 (AGO1) protein to regulate target gene expression primarily through mRNA cleavage. We report here the discovery of a new class of miRNAs in the model monocot rice (Oryza sativa). These are 24 nt in length and require another member of the Dicer family, DCL3, for their biogenesis. The 24 nt long miRNAs (lmiRNAs) are loaded into A...

Provenance — who produced it, who reused it

Linked to 4 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Liang WuHuanyu ZhouQingqing ZhangYijun Qi
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

48/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Oryza sativa
Instrument
Illumina Genome Analyzer
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
7 / 7 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 2095515740 reported
total reads 58808534 reported
n content pct 0.021 measured
pct q20 bases 95 measured
pct q30 bases 77.1 measured
gc content pct 49.3 measured
mean read length 36 measured
mean base quality 30.2 measured
adapter content pct 0 measured
duplication rate pct 54.43 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 48/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 77.1 measured ×1 36%
mean base quality 30.2 measured ×0.6 37%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 54.43 measured ×0.4 46%
QC cost 22 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0