Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE29422

GEO first seen 2016

Chromatin and Transcriptional Signatures for Nodal Signaling During Endoderm Formation in hESCs

Organism
Homo sapiens
Samples
18
Type
Genome binding/occupancy pro...
Submitted
2011-05-20

To elucidate the Nodal transcriptional network that governs endoderm formation, we used ChIP-Seq to identify genomic targets for SMAD2/3, SMAD3, SMAD4, FOXH1 and the active and repressive chromatin marks, H3K4me3 and H3K27me3, in human embryonic stem cells (hESCs) and derived endoderm. We demonstrate that while SMAD2/3, SMAD4 and FOXH1 target binding is highly dynamic, there is an optimal signature for driving endoderm commitment. Initially, this signature is marked by both H3K4me3 and H3K27me3...

Provenance — who produced it, who reused it

Linked to 12 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Si W KimSe-Jin YoonEdward ChuongChuba OyoluJulie Baker
Reused by

9 further papers cite this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Homo sapiens
Files available
BED, TXT, WIG
Metrics (value · how obtained)
n content pct 0.002 measured
pct q20 bases 93.5 measured
pct q30 bases 70.2 measured
gc content pct 49.7 measured
mean read length 36 measured
mean base quality 29.5 measured
adapter content pct 0.01 measured
duplication rate pct 2.46 measured
supplementary file types BED, TXT, WIG reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 70.2 measured ×1 1%

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 100