Digital gene expression and global mapping of polyadenylation sites with PolyA-Seq
We describe PolyA-Seq, a strand-specific method for high-throughput sequencing of the 3' ends of polyadenylated transcripts. PolyA-Seq is as accurate for digital gene expression as existing RNA sequencing approaches, and superior to microarrays. We used the approach to map polyadenylation (polyA) sites in 24 samples from normal tissues in human, rhesus, dog, mouse, and rat.
Provenance — who produced it, who reused it
Linked to 13 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- A comprehensive analysis of 3′ end sequencing data sets reveals... 2016 · 237 cites
- Creating reference gene annotation for the mouse C57BL6/J genome... 2015 · 200 cites
- hnRNPC regulates cancer-specific alternative cleavage and polyad... 2019 · 143 cites
- Exon-Mediated Activation of Transcription Starts 2019 · 109 cites
8 further papers cite this accession but reuse could not be confirmed.
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently