Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE30400

GEO first seen 2015

RNA-Seq in GM12878 (ENCODE Project)

Organism
Homo sapiens
Samples
1
Type
Expression profiling by high...
Submitted
2011-07-05

We performed RNA-Seq as part of the ENCODE project in order to determine sites of allele-specific expression This was done in the GM12878 cell line which was genotyped as part of the pilot II phase of the 1000 genomes project. There is a matching ChIP-Seq experiments for a number of transcription factors were also performed on the same cell line. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleaseP...

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Joel RozowskyDebahish RahaMichael SnyderMark Gerstein
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.675 measured
pct q20 bases 35.5 measured
pct q30 bases 14.7 measured
gc content pct 47 measured
mean read length 54 measured
mean base quality 12.4 measured
adapter content pct 0.41 measured
duplication rate pct 2.31 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 14.7 measured ×1 0%
mean base quality 12.4 measured ×0.6 0%
adapter content pct 0.41 measured ×0.4 100%
duplication rate pct 2.31 measured ×0.4 100%
QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0