Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE32916

GEO first seen 2012

Analysis of Pro-Inflammatory Gene Activation and RNA Processing by RNAseq of Nascent Transcripts I

Organism
Mus musculus
Samples
15
Type
Expression profiling by high...
Submitted
2011-10-12

We examined pro-inflammatory gene activation in activated murine macrophages by performing RNA-Seq with fractionated chromatin-associated, nucleoplasmic, and cytoplasmic transcripts. This experimental strategy allowed a global, high-resolution analysis of the transcriptional regulation of diverse classes of co-expressed genes, with a direct comparison to transcript processing and the transit of RNA from the chromatin to the nucleoplasm and the cytoplasm. The results provide quantitative insights...

Provenance — who produced it, who reused it

Linked to 8 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

52/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.347 measured
pct q20 bases 91.9 measured
pct q30 bases 91.9 measured
gc content pct 56.8 measured
mean read length 50 measured
mean base quality 27.8 measured
adapter content pct 8.45 measured
duplication rate pct 76.47 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 52/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.9 measured ×1 100%
mean base quality 27.8 measured ×0.6 0%
adapter content pct 8.45 measured ×0.4 64%
duplication rate pct 76.47 measured ×0.4 0%
QC cost 28 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0