Pyrococcus furiosus crRNAs associated with the Cas RAMP module complex
Analysis of CRISPR RNAs from both total Pyrococcus furiosus RNA and those that co-purify with the Cmr complex, which has been predicted to be involved in RNA-mediated targeting of foreign RNAs in prokaryotes. Small RNAs (20 - 70 nts) were isolated either from total Pyrococcus furiosus RNA or RNAs isolated from immunopurified Cmr complexes. The RNAs were cloned and sequenced using the Illumina platform. Results provide insight into the anatomy of Cmr2-associated CRISPR RNAs.
Provenance — who produced it, who reused it
Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Obesity-associated NLRC4 inflammasome activation drives breast c... 2016 · 240 cites
1 further paper cites this accession but reuse could not be confirmed.
Deep data QC
69/100 · DStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0