Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE35496

GEO first seen 2013

ChIP-Seq of Sox2 and Brn2 in ESCs, NPCs, and differentiating ESCs

Organism
Mus musculus
Samples
30
Type
Genome binding/occupancy pro...
Submitted
2012-02-02

We analyzed the genome-wide binding of Sox2 and POU factor partner factors, Oct4 in ESCs (using published datasets PMID:18692474 and GSM307137, GSM307154, GSM307155) and Brn2 in NPCs. We found that Sox2 and Oct4 co-occupied a large subset of promoters and enhancers in ESCs, but that Sox2 and Brn2 co-occupy predominantly enhancers. Further, we overexpressed Brn2 in differentiating ESCs and showed that ectopic Brn2 recruited Sox2 to NPC-specific targets, resulting in skewed differentiation toward...

Provenance — who produced it, who reused it

Linked to 10 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Michael A LodatoRudolf JaenischLaurie A Boyer
Reused by

7 further papers cite this accession but reuse could not be confirmed.

Deep data QC

metadata only · no data-level QC for this type

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Mus musculus
Instrument
Illumina Genome Analyzer II
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
30 / 30 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 35186829464 reported
total reads 886997801 reported
supplementary file types WIG reported
QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently