Tracing pluripotency of human early embryos and embryonic stem cells by single cell RNA-seq
Find the casual relationship between gene expression network and cellular phenotype at single cell resolution. We collected donated human pre-implatation embryos, and the embryonic stem cells derived from them, isolate individual cells, prepared single cell cDNAs, and sequenced them by HiSeq2000. Then we analyzed the expression of known RefSeq genes.
Provenance — who produced it, who reused it
Linked to 74 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- DNA methylation dynamics of the human preimplantation embryo 2014 · 586 cites
- Single-cell entropy for accurate estimation of differentiation p... 2017 · 377 cites
- Hominoid-Specific Transposable Elements and KZFPs Facilitate Hum... 2019 · 322 cites
- Tunable protein synthesis by transcript isoforms in human cells 2016 · 304 cites
- Pluripotent stem cell-derived model of the post-implantation hum... 2023 · 224 cites
- Dynamics and clinical relevance of maternal mRNA clearance durin... 2020 · 209 cites
- scMerge leverages factor analysis, stable expression, and pseudo... 2019 · 207 cites
- Specific gene-regulation networks during the pre-implantation de... 2014 · 170 cites
- Cell lineage and communication network inference via optimizatio... 2019 · 166 cites
- Multi-omic Profiling Reveals Dynamics of the Phased Progression... 2019 · 162 cites
- Principles of signaling pathway modulation for enhancing human n... 2021 · 162 cites
- 8C-like cells capture the human zygotic genome activation progra... 2022 · 156 cites
- scEpath: energy landscape-based inference of transition probabil... 2018 · 143 cites
- Linnorm: improved statistical analysis for single cell RNA-seq e... 2017 · 134 cites
- SC3-seq: a method for highly parallel and quantitative measureme... 2015 · 128 cites
- Early X chromosome inactivation during human preimplantation dev... 2017 · 111 cites
- Single-cell CAS-seq reveals a class of short PIWI-interacting RN... 2019 · 110 cites
- Benchmark and Parameter Sensitivity Analysis of Single-Cell RNA... 2019 · 86 cites
- Evolutionary transition between invertebrates and vertebrates vi... 2019 · 78 cites
- Transcriptome-wide Variability in Single Embryonic Development C... 2014 · 77 cites
- The landscape of human mutually exclusive splicing 2017 · 75 cites
- HEMO, an ancestral endogenous retroviral envelope protein shed i... 2017 · 74 cites
52 further papers cite this accession but reuse could not be confirmed.
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
No quantitative QC rubric exists for this data type yet, so it is deliberately left unscored — this is an honest "not applicable", not a poor rating.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently
Scientific quality
Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.