Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE39232

GEO first seen 2013

The maize methylome modulates mRNA splicing and reveals widespread paramutation guided by small RNA.

Organism
Zea mays
Samples
35
Type
Expression profiling by high...
Submitted
2012-07-10

Methylation of chromosomal DNA in animals and plants is a fundamental mechanism of epigenetic regulation, and the maize genome, with its diverse complement of transposons and repeats, is a paradigm for transgenerational mechanisms such as paramutation and imprinting. We have determined the genome-wide cytosine methylation map of two maize inbred lines, B73 and Mo17, at high coverage and at single nucleotide resolution. Transposon methylation is highest in CG (65%) and CHG (50%) contexts (where H...

Provenance — who produced it, who reused it

Linked to 7 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Michael RegulskiZhenyuan LuJude KendallJon ReindersVictor LlacaStephane DeschampsAndrew SmithDan LevyW R McCombieScott TingeyAntoni RafalskiJim HicksDoreen WareRobert Martienssen
Reused by

5 further papers cite this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
methylation
Organism
Zea mays
Instrument
Illumina Genome Analyzer
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
143 / 35 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 572130270457 reported
total reads 6017002485 reported
n content pct 0.047 measured
pct q20 bases 99.3 measured
pct q30 bases 98.2 measured
gc content pct 52.3 measured
mean read length 50 measured
mean base quality 39 measured
adapter content pct 0.34 measured
duplication rate pct 43.32 measured
supplementary file types BED, TXT reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published methylation thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 98.2 measured ×1 100%
QC cost 33 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0