Evolutionary dynamics of gene and isoform regulation in mammalian tissues
Most mammalian genes produce multiple distinct mRNAs through alternative splicing, but the extent of splicing conservation is not clear. To assess tissue-specific transcriptome variation across mammals, we sequenced cDNA from 9 tissues from 4 mammals and one bird in biological triplicate, at unprecedented depth. We find that while tissue-specific gene expression programs are largely conserved, alternative splicing is well conserved in only a subset of tissues and is frequently lineage-specific...
Provenance — who produced it, who reused it
Linked to 15 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Annotation-free quantification of RNA splicing using LeafCutter 2017 · 847 cites
- Distinct longevity mechanisms across and within species and thei... 2023 · 149 cites
- Exon-Mediated Activation of Transcription Starts 2019 · 109 cites
12 further papers cite this accession but reuse could not be confirmed.
Deep data QC
88/100 · BStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Mouse bulk RNA-seq. Grade B (provisional). Solid measured Q30 (87.9%) and base quality (34) are offset by moderate duplication (51.49%) suggesting PCR bias; however, metadata fetch error prevents full verification. Deep measured re-pass is pending.
The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0
Scientific quality
Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.