Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE45719

GEO first seen 2014

Single-cell RNA-Seq reveals dynamic, random monoallelic gene expression in mammalian cells

Organism
Mus musculus
Samples
317
Type
Expression profiling by high...
Submitted
2013-04-02

In the diploid genome, genes come in two copies, which can have different DNA sequence and where one is maternal and one is paternal. In a particular cell, a gene could potentially be expressed from both copies (biallelic expression) or only one (monoallelic). We performed RNA-Sequencing on individual cells, from zygote to the cells of the late blastocyst, and also individual cells from the adult liver. Using first generation crosses between two distantly related mouse strains, CAST/Ei and C57BL...

Provenance — who produced it, who reused it

Linked to 48 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Qiaolin DengDaniel RamsköldBjörn ReiniusRickard Sandberg
Reused by

34 further papers cite this accession but reuse could not be confirmed.

Deep data QC

95/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
317 / 317 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 379502668441 reported
total reads 7143537531 reported
n content pct 0 measured
pct q20 bases 99.1 measured
pct q30 bases 97.2 measured
gc content pct 48.2 measured
mean read length 43 measured
mean base quality 37.7 measured
adapter content pct 0.02 measured
duplication rate pct 43.42 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 95/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.2 measured ×1 100%
mean base quality 37.7 measured ×0.6 100%
adapter content pct 0.02 measured ×0.4 100%
duplication rate pct 43.42 measured ×0.4 70%
QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0