Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE46224

GEO first seen 2014

Deep RNA Sequencing Reveals Dynamic Regulation of Myocardial Noncoding RNA in Failing Human Heart and Remodeling with Mechanical Circulatory Support

Organism
Homo sapiens
Samples
80
Type
Non-coding RNA profiling by...
Submitted
2013-04-19

Complete transcriptome profiling in human failing and non-failing control hearts using next-gen sequencing

Provenance — who produced it, who reused it

Linked to 20 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Kai-Chien YangJeanne Nerbonne
Reused by

18 further papers cite this accession but reuse could not be confirmed.

Deep data QC

97/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
80 / 80 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 142609539182 reported
total reads 1108781515 reported
n content pct 0.047 measured
pct q20 bases 97.9 measured
pct q30 bases 94.4 measured
gc content pct 49.5 measured
mean read length 101 measured
mean base quality 36.2 measured
adapter content pct 0.13 measured
duplication rate pct 38.83 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 97/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.4 measured ×1 100%
mean base quality 36.2 measured ×0.6 100%
adapter content pct 0.13 measured ×0.4 100%
duplication rate pct 38.83 measured ×0.4 80%
QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0