Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position
We describe an assay for transposase-accessible chromatin using sequencing (ATAC-seq), based on direct in vitro transposition of sequencing adaptors into native chromatin, as a rapid and sensitive method for integrative epigenomic analysis. ATAC-seq captures open chromatin sites using a simple two-step protocol with 500–50,000 cells and reveals the interplay between genomic locations of open chromatin, DNA-binding proteins, individual nucleosomes and chromatin compaction at nucleotide resolution...
Provenance — who produced it, who reused it
Linked to 31 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- An improved ATAC-seq protocol reduces background and enables int... 2017 · 2,749 cites
- Identification of transcription factor binding sites using ATAC-... 2019 · 510 cites
- Simultaneous profiling of chromatin accessibility and methylatio... 2020 · 245 cites
- Cell‐free DNA as a biomarker of aging 2018 · 150 cites
- Single-cell lineage tracing by endogenous mutations enriched in... 2019 · 148 cites
- ATAC-seq normalization method can significantly affect different... 2020 · 106 cites
- Multiomic Profiling Identifies cis-Regulatory Networks Underlyin... 2019 · 82 cites
23 further papers cite this accession but reuse could not be confirmed.
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently