Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE48968

GEO first seen 2014

Single-cell RNA-seq reveals dynamic paracrine control of cellular variation

Organism
Mus musculus
Samples
2,425
Type
Expression profiling by high...
Submitted
2013-07-17

Here, we use a microfluidics-based approach to prepare single-cell RNA-Seq libraries from over 1,700 primary mouse dendritic cells (DCs) stimulated with three pathogenic components and examine variation between individual cells exposed to the same stimulus and general strategies that multicellular populations use to establish complex dynamic responses.

Provenance — who produced it, who reused it

Linked to 18 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

13 further papers cite this accession but reuse could not be confirmed.

Deep data QC

90/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.002 measured
pct q20 bases 97.1 measured
pct q30 bases 92.1 measured
gc content pct 47 measured
mean read length 125 measured
mean base quality 35.4 measured
adapter content pct 8.43 measured
duplication rate pct 40.74 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 90/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.1 measured ×1 100%
mean base quality 35.4 measured ×0.6 100%
adapter content pct 8.43 measured ×0.4 64%
duplication rate pct 40.74 measured ×0.4 76%
QC cost 35 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0