Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE50760

GEO first seen 2016

Gene expression profiling study by RNA-seq in colorectal cancer

Organism
Homo sapiens
Samples
54
Type
Expression profiling by high...
Submitted
2013-09-11

The objective of this study is to identify a prognostic signature in colorectal cancer (CRC) patients with diverse progression and heterogeneity of CRCs. We generated RNA-seq data of 54 samples (normal colon, primary CRC, and liver metastasis) from 18 CRC patients and, from the RNA-seq data, identified significant genes associated with aggressiveness of CRC. Through diverse statistical methods including generalized linear model likelihood ratio test, two significantly activated regulators were i...

Provenance — who produced it, who reused it

Linked to 39 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Jeong-Hwan KimSeon-Kyu KimSeon-Young KimJin-Cheon Kim
Reused by

34 further papers cite this accession but reuse could not be confirmed.

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
54 / 54 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 331188330986 reported
total reads 1639546193 reported
n content pct 0.015 measured
pct q20 bases 77.2 measured
pct q30 bases 67.3 measured
gc content pct 52 measured
mean read length 101 measured
mean base quality 28 measured
adapter content pct 0.3 measured
duplication rate pct 12.72 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 67.3 measured ×1 0%
mean base quality 28 measured ×0.6 0%
adapter content pct 0.3 measured ×0.4 100%
duplication rate pct 12.72 measured ×0.4 100%
QC cost 28 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0