Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE52564

GEO first seen 2016

An RNA-Seq transcriptome and splicing database of neurons, glia, and vascular cells of the cerebral cortex

Organism
Mus musculus
Samples
17
Type
Expression profiling by high...
Submitted
2013-11-20

Purpose: To better understand the function of the various cell types of the brain, we prospectively purified neurons, astrocytes, oligodendrocyte precursor cells, newly formed oligodendrocytes, myelinating oligodendrocytes, microglia, endothelial cells, and pericytes from mouse cerebral cortex. We generated a transcriptome database for these 8 cell types by RNA sequencing and used a highly sensitive algorithm to detect alternative splicing events in each gene. Our analysis identified thousands o...

Provenance — who produced it, who reused it

Linked to 35 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Ye ZhangKenian ChenSteven A SloanAnja R ScholzeChristine CanedaNadine RuderischShuyun DengRichard DanemanBen A BarresJia Q Wu
Reused by

23 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
17 / 17 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 110406582884 reported
total reads 546567242 reported
n content pct 0.012 measured
pct q20 bases 97.5 measured
pct q30 bases 94.3 measured
gc content pct 49.1 measured
mean read length 101 measured
mean base quality 36.5 measured
adapter content pct 0.83 measured
duplication rate pct 7.08 measured
supplementary file types XLS reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.3 measured ×1 100%
mean base quality 36.5 measured ×0.6 100%
adapter content pct 0.83 measured ×0.4 100%
duplication rate pct 7.08 measured ×0.4 100%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0