Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE52628

GEO first seen 2015

Genome-wide map of H3K4me3 in 12 day post-partum mouse spermatocytes

Organism
Mus musculus
Samples
14
Type
Genome binding/occupancy pro...
Submitted
2013-11-21

Here we characterize the genome-wide chromatin modification by PRDM9, a histone H3 lysine 4 methyltransferase. In order to detect PRDM9 binding sites we created coisogenic strains of mice differing only in the zinc finger array of PRDM9. One strain is C57BL/6J, which carries the Prdm9Dom2 allele, the other strain was created using genomic replacement and named B6.PRDM9Cst (also called KI), and contains the Prdm9Cst allele originally found in CAST/EiJ mice. Many H3K4me3 positions are common betwe...

Provenance — who produced it, who reused it

Linked to 10 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Christopher L BakerKenneth Paigen
Reused by

6 further papers cite this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
16 / 14 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 77994817313 reported
total reads 777531372 reported
n content pct 0.001 measured
pct q20 bases 94.7 measured
pct q30 bases 88 measured
gc content pct 48.9 measured
mean read length 100 measured
mean base quality 34.7 measured
adapter content pct 1.42 measured
duplication rate pct 4.79 measured
supplementary file types BED, BEDGRAPH, CSV, TXT reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88 measured ×1 90%
QC cost 11 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0